Virologie a diagnostika
Vedoucí oddělení: Dr. Milan Fránek, DrSc.
telefon: 533 331 901e-mail: franek__(at)__vri.cz
Oddělení je zaměřeno na vývoj diagnostiky a prevence významných infekčních chorob skotu a prasat a na vývoj metod k detekci kontaminace potravin a životního prostředí. Skupina virové choroby skotu a skupina virové choroby prasat jsou zaměřeny na studium patogeneze, na vývoj nových diagnostik a vakcín (IBR, BVD, BRSV,PI3) a na sestavování ozdravovacích programů infekčních chorob. Transfer nových výsledků a technologií do praxe je realizován zejména ve spolupráci s chovatelskými svazy, SVS a MZe (ozdravovací programy) a s komerčními výrobci diagnostik a biopreparátů (Test-Line, s.r.o., Bioveta a.s.). Pracovní skupina analytická biotechnologie je orientována na výzkum, vývoj a inovaci metod, které umožňují zavedení nových analytických a diagnostických postupů, na kontrolu (monitorování) nezávadnosti a kvality potravin, krmiv, vody, půdy a dalších komponent potravního řetězce a životního prostředí. Transfer nových analytických a diagnostických technologií je prováděn licencováním těchto produktů výzkumu s komerčními výrobci (R-Biopharm, Abraxis LLC, Bioo Scientific, SEDIUM RD s.r.o.).
Publikace za poslední tři roky
Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians
Cinkova K, Reschova S, Kulich P, Vesely TDiseases of Aquatic Organisms 89, 2010, 191-198
Effect of Sodium Humate on the Content of Trace Elements in Organs of Weaned Piglets
Zraly Z, Pisarikova BActa Veterinaria Brno 79, 2010, 73-79
Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic
Ficek R, Psikal I, Fictum P, Bendova J, Kosinova E, Smitalova R, Skoric MActa Veterinaria Brno 79, 2010, 81-90
Characterization of two BHV-4 strains isolated in the Czech Republic
Fichtelova V, Kovarcik KVeterinarni Medicina 55, 2010, 106-112
http://vri.cz/docs/vetmed/55-3-106.pdfHerpesvirus-Associated Papillomatosis in a Green Lizard
Literak L, Robesova B, Majlathova V, Majlath I , Kulich P, Fabian P, Roubalova EJournal of Wildlife Diseases 46, 2010, 257-261
Detection and genetic characterisation of Hepatitis E virus in Czech pig production herds
Vasickova P, Psikal I, Widen F, Smitalova R, Bendova J, Pavlik I, Kralik PResearch in Veterinary Science 87, 2009, 143-148
Heterologous expression and molecular characterization of the NAD(P)H:acceptor oxidoreductase (FerB) of Paracoccus denitrificans
Tesarik R, Sedlacek V, Plockova J, Wimmerova M , Turanek J, Kucera IProtein Expression and Purification 68, 2009, 233-238
In-house validation of liquid chromatography tandem mass spectrometry for determination of semicarbazide in eggs and stability of analyte in matrix
Stastny K, Frgalova K, Hera A, Vass M, Franek MJournal of Chromatography A 1216, 2009, 8187-8191
Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic
Smitalova R, Rodak L, Smid B, Psikal IVeterinarni Medicina 54, 2009, 12-18
http://www.vri.cz/docs/vetmed/54-1-12.pdfGenotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea
Prodelalova J, Malenovska H, Valicek LVeterinarni Medicina 54, 2009, 461-466
http://www.vri.cz/docs/vetmed/54-10-461.pdfGenotyping of bovine viral diarrhoea virus isolates from the Czech Republic
Robesova B, Kovarcik K, Vilcek SVeterinarni Medicina 54, 2009, 393-398
http://www.vri.cz/docs/vetmed/54-9-393.pdfSperm and Embryo Analysis in a Carrier of Supernumerary inv dup(15) Marker Chromosome
Oracova E, Musilova P, Kopecna O, Rybar R, Vozdova M, Vesela K, Rubes JJournal of Andrology 30, 2009, 233-239
Heterologous expression of full-length capsid protein of porcine circovirus 2 in Escherichia coli and its potential use for detection of antibodies
Marcekova Z, Psikal I, Kosinova E, Benada O, Sebo P, Bumba LJournal of Virological Methods 162, 2009, 133-141
Rapid Alert System for Food and Feed: The semicarbazide notifications
Hruska K, Franek MVeterinarni Medicina 54, 2009, 561-564
http://www.vri.cz/docs/vetmed/54-11-561.pdfBiolabeling with 2,4-Dichlorophenoxyacetic Acid Derivatives: The 2,4-D Tag
Bade S, Rockendorf N, Franek M, Gorris HH, Lindner B, Olivier V, Schaper KJ, Frey AAnalytical Chemistry 81, 2009, 9695-9702
Rapid Profiling of Peptide Stability in Proteolytic Environments
Gorris HH, Bade S, Rockendorf N, Albers E, Schmidt MA, Franek M, Frey AAnalytical Chemistry 81, 2009, 1580-1586
Nitrofuran antibiotics: a review on the application, prohibition and residual analysis
Vass M, Hruska K, Franek MVeterinarni Medicina 53, 2008, 469-500
http://www.vri.cz/docs/vetmed/53-9-469.pdfELISA for semicarbazide and its application for screening in food contamination
Vass M, Diblikova I, Cernoch I, Franek MAnalytica Chimica Acta 608, 2008, 86-94
In-house validation of an ELISA method for screening of semicarbazide in eggs
Vass M, Diblikova I, Kok E, Stastny K, Frgalova K, Hruska K, Franek MFood Additives and Contaminants 25, 2008, 930-936
Serological detection of Trichinella spiralis in swine by ELISA (enzyme-linked immunosorbent assay) using an excretory-secretory (E/S) antigen
Korinkova K, Kovarcik K, Pavlickova Z, Svoboda M , Koudela BParasitology Research 102, 2008, 1317-1320
Characterisation of immunosuppression in rabbits after infection with myxoma virus
Jeklova E, Leva L, Matiasovic J, Kovarcik K, Kudlackova H, Nevorankova Z, Psikal I, Faldyna MVeterinary Microbiology 129, 2008, 117-130
Comparative inactivation of Aujeszky's disease virus, Porcine teschovirus and Vesicular stomatitis virus by chemical disinfectants
Dvorakova H, Prodelalova J, Reichelova MVeterinarni Medicina 53, 2008, 236-242
http://www.vri.cz/docs/vetmed/53-5-236.pdfDevelopment of an ELISA-based kit for the on-farm determination of progesterone in milk
Simersky R, Swaczynova J, Morris DA, Franek M, Strnad MVeterinarni Medicina 52, 2007, 19-28
http://www.vri.cz/docs/vetmed/52-1-19.pdfDetection of spring viraemia of carp virus (SVCV) with monoclonal antibodies
Reschova S, Pokorova D, Nevorankova Z, Hulova J , Vesely TVeterinarni Medicina 52, 2007, 308-316
http://www.vri.cz/docs/vetmed/52-7-308.pdfHumoral immune responses during experimental infection with Fascioloides magna and Fasciola hepatica in goats and comparison of their excretory/secretory products
Novobilsky A, Kasny M, Mikes L, Kovarcik K, Koudela BParasitology Research 101, 2007, 357-364
Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay
Kandimalla VB, Kandimalla N, Hruska K, Franek MVeterinarni Medicina 52, 2007, 445-450
http://www.vri.cz/docs/vetmed/52-10-445.pdfReal-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
Kosinova E, Psikal I, Robesova B, Kovarcik KVeterinarni Medicina 52, 2007, 253-261
http://www.vri.cz/docs/vetmed/52-6-253.pdfA correlation of in vitro tests for the immune response detection: A bovine trichophytosis model
Faldyna M, Oborilova E, Krejci J, Tesarik R, Krejci E, Pavlova B, Rybnikar AVaccine 25, 2007, 7948-7954