Food and feed safety
Head of the department: Prof. MVDr. Ivo Pavlík, CSc.
phone: +420 533 331 601
e-mail: pavlik__(at)__vri.cz
The Department of Food and Feed Safety consists of more than 30 scientists who are involved in research and consultative activities in various food and feed safety topics. Research activities cover mainly bacterial and viral pathogens with zoonotic potential, food microbiology, feed safety and animal nutrition. The department is equipped with modern laboratory facilities, which are used for the development of new diagnostic methods, approaches and recommendations, identification of viral or bacterial agents from various biological and environmental samples, epidemiologic and pathogenesis studies and testing of antimicrobial and stress resistance of bacteria. Additionally, the Department of Food and Feed Safety participates in the education of students at the Veterinary and Pharmaceutical University Brno, Mendel University in Brno and Faculty of Science in Masaryk University Brno.
The department consists of five research groups in which two OIE reference laboratories and three Methodical and Consultation Centres (MCC) are included.
Reference Laboratories and Methodical and Consultation Centres (MCC) for the State Veterinary Administration of the Czech Republic
OIE Reference Laboratory for Paratuberculosis (Prof. MVDr. Ivo Pavlík, CSc.)
OIE Reference Laboratory for Avian Tuberculosis (Prof. MVDr. Ivo Pavlík, CSc.)
MCC for tuberculosis, paratuberculosis and mycobacteriosis of animals (Prof. MVDr. Ivo Pavlík, CSc.)
MCC for study of nutrition impact on animal health (Ing. Martina Trčková, PhD.)
MCC for antimicrobial resistance of microorganisms (DVM. Zoran Jaglič, PhD.)
Research groups:
Tuberculosis, Paratuberculosis and Mycobacteriosis of Animals – head Prof. MVDr. Ivo Pavlík, CSc.
Molecular Mycobacteriology – head Mgr. Iva Slaná, PhD.
Animal Nutrition and Feed Safety – head Ing. Martina Trčková, PhD.
Food Microbiology – head DVM. Zoran Jaglič, PhD.
Food Virology – head Mgr. Petra Vašíčková
The group of Tuberculosis, Paratuberculosis and Mycobacteriosis of animals
e-mail: pavlik__(at)__vri.cz
The Department of Food and Feed Safety consists of more than 30 scientists who are involved in research and consultative activities in various food and feed safety topics. Research activities cover mainly bacterial and viral pathogens with zoonotic potential, food microbiology, feed safety and animal nutrition. The department is equipped with modern laboratory facilities, which are used for the development of new diagnostic methods, approaches and recommendations, identification of viral or bacterial agents from various biological and environmental samples, epidemiologic and pathogenesis studies and testing of antimicrobial and stress resistance of bacteria. Additionally, the Department of Food and Feed Safety participates in the education of students at the Veterinary and Pharmaceutical University Brno, Mendel University in Brno and Faculty of Science in Masaryk University Brno.The department consists of five research groups in which two OIE reference laboratories and three Methodical and Consultation Centres (MCC) are included.
Reference Laboratories and Methodical and Consultation Centres (MCC) for the State Veterinary Administration of the Czech Republic
OIE Reference Laboratory for Paratuberculosis (Prof. MVDr. Ivo Pavlík, CSc.)
OIE Reference Laboratory for Avian Tuberculosis (Prof. MVDr. Ivo Pavlík, CSc.)
MCC for tuberculosis, paratuberculosis and mycobacteriosis of animals (Prof. MVDr. Ivo Pavlík, CSc.)
MCC for study of nutrition impact on animal health (Ing. Martina Trčková, PhD.)
MCC for antimicrobial resistance of microorganisms (DVM. Zoran Jaglič, PhD.)
Research groups:
Tuberculosis, Paratuberculosis and Mycobacteriosis of Animals – head Prof. MVDr. Ivo Pavlík, CSc.
Molecular Mycobacteriology – head Mgr. Iva Slaná, PhD.
Animal Nutrition and Feed Safety – head Ing. Martina Trčková, PhD.
Food Microbiology – head DVM. Zoran Jaglič, PhD.
Food Virology – head Mgr. Petra Vašíčková
The group of Tuberculosis, Paratuberculosis and Mycobacteriosis of animals
• Collects and processes samples for cultivation and culture independent methods • Identifies mycobacteria using culture and Ziehl-Neelsen microscopy
• Diagnoses presence of selected mycobacteria using serological methods (e.g. MAPIA, ELISA test for selected mycobacteria, agglutination for M. avium complex infections in birds and pigs)
• Performs basic research on epidemiology and environmental mycobacteriology
The Molecular Mycobacteriology group is specialised in
• Development of culture independent methods (DNA and RNA isolation, PCR, sequencing) for identification of Mycobacterium sp. • Examination of biological samples for the presence of Mycobacterium sp. using culture independent methods
• Epidemiological studies on selected mycobacteria (Mycobacterium avium subsp. avium, Mycobacterium avium subsp. paratuberculosis, Mycobacterium avium subsp. hominissuis) using RFLP analysis
• Study of gene expression in Mycobacterium avium subsp. paratuberculosis as a response on stress factors
The aim of the Animal Nutrition and Feed Safety group is to
• Verify suitability of various non-nutritive feeding additives for livestock • Verify the effect of non-nutritive feeding additives and their influence to animal’s health
• Analyse feeding mixtures
The Food Microbiology group is focused on
• Surveillance of bacterial contamination in raw food material, food products and food processing equipment • Monitoring of antimicrobial resistance of selected bacteria
• Research in the area of bacterial biofilms and resistance to disinfectant agents
Main activities of the Food Virology group include
• Development of cell-culture independent methods (RNA and DNA isolation, RT-PCR, PCR) for identification of food-borne viruses (Genera: Adenovirus, Norovirus, Hepavirus and Hepevirus) in biological and environmental samples • Collection of samples from vegetable and animal food product chains, and the detection of food-borne viruses in these samples
• Detection of hepatitis E virus in biological samples of domestic and wild animals, and in clinical samples of human patients
Selected publications:
Ayele, W.Y., Svastova, P., Roubal, P., Bartos, M., Pavlik, I.: Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic. Appl.Environ.Microbiol., 2005, 71 (3), 1210-1214.
Babak, V., Schlegelova, J., Vlkova, H.: Interpretation of the results of antimicrobial susceptibility analysis of Escherichia coli isolates from bovine milk, meat and associated foodstuffs. Food Microbiology, 2005, 22 (4), 353-358.
Bartos, M., Hlozek, P., Svastova, P., Dvorska, L., Bull, T., Matlova, L., Parmova, I., Kuhn, I., Stubbs, J., Moravkova, M., Kintr, J., Beran, V., Melicharek, I., Ocepek, M., Pavlik, I.: Identification of members of Mycobacterium avium species by Accu-Probes, serotyping, and single IS900, IS901, IS1245 and IS901-flanking region PCR with internal standards. Journal of Microbiological Methods, 2006, 64 (3), 333-345.
Beran, V., Matlova, L., Dvorska, L., Svastova, P., Pavlik, I.: Distribution of mycobacteria in clinically healthy ornamental fish and their aquarium environment. Journal of Fish Diseases, 2006, 29 (7), 383-393.
Beran, V., Havelkova, M., Kaustova, J., Dvorska, L., Pavlik, I.: Cell wall deficient forms of mycobacteria: a review. Veterinarni Medicina, 2006, 51 (7), 365-389.
Cvetnic, Z., Spicic, S., Katalinic-Jankovic, V., Marjanovic, S., Obrovac, M., Benic, M., Mitak, M., Pavlik, I.: Mycobacterium caprae infection in cattle and pigs on one family farm in Croatia: a case report. Veterinarni Medicina, 2006, 51 (11), 523-531.
Djonne, B., Pavlik, I., Svastova, P., Bartos, M., Holstad, G.: IS900 restriction fragment length polymorphism (RFLP) analysis of Mycobacterium avium subsp paratuberculosis isolates from goats and cattle in Norway. Acta Veterinaria Scandinavica, 2005, 46 (1-2), 13-18.
Dvorska, L., Matlova, L., Ayele, W.Y., Fischer, O.A., Amemori, T., Weston, R.T., Alvarez, J., Beran, V., Moravkova, M., Pavlik, I.: Avian tuberculosis in naturally infected captive water birds of the Ardeideae and Threskiornithidae families studied by serotyping, IS901 RFLP typing, and virulence for poultry. Veterinary Microbiology, 2007, 119 (2-4), 366-374.
Gazouli, M., Ikonomopoulos, J., Koundourakis, A., Bartos, M., Pavlik, I., Overduin, P., Kremer, K., Gorgoulis, V., Kittas, C.: Characterization of Mycobacterium tuberculosis complex isolates from Greek patients with sarcoidosis by spoligotyping. Journal of Clinical Microbiology, 2005, 43 (9), 4858-4861.
Herzig, I., Travnicek, J., Kursa, J., Kroupova, V.: The content of iodine in pork. Veterinarni Medicina, 2005, 50 (12), 521-525.
Herzig, I., Navratilova, M., Suchy, P., Vecerek, V., Totusek, J.: Model trial investigating retention in selected tissues using broiler chicken fed cadmium and humic acid. Veterinarni Medicina, 2007, 52 ( 4), 162-168.
Herzig, I., Travnicek, J., Kursa, V., Kroupova, J., Reznicek, I.: Content of iodine in broiler meat. Acta Veterinaria Brno, 2007, 76 (1), 137-141.
Holasova, M., Karpiskova, R., Karpiskova, S., Babak, V., Schlegelova, J.: Comparison of methods for the determination of antimicrobial resistance in Campylobacter spp. human and the food chain isolates. Veterinarni Medicina, 2007, 52 (4), 169-174.
Hruska, K., Bartos, M., Kralik, P., Pavlik, I.: Mycobacterium avium subsp paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved. Veterinarni Medicina, 2005, 50 (8), 327-335.
Ikonomopoulos, J., Pavlik, I., Bartos, M., Svastova, P., Ayele, W.Y., Roubal, P., Lukas, J., Cook, N., Gazouli, M.: Detection of Mycobacterium avium subsp paratuberculosis in retail cheeses from Greece and the Czech republic. Applied and Environmental Microbiology, 2005, 71 (12), 8934-8936.
Ikonomopoulos, J., Balaskas, C., Kantzoura, B., Fragiadaki, E., Pavlik, I., Bartos, M., Lukas, J.C., Gazouli, M.: Comparative evaluation of positive tests to Mycobacterium avium subsp paratuberculosis in clinically healthy sheep and goats in South-West Greece using molecular techniques, serology, and culture. Veterinary Journal, 2007, 174 (2), 337-343.
Jesenska, A., Pavlova, M., Strouhal, M., Chaloupkova, R., Tesinska, I., Monincova, M., Prokop, Z., Bartos, M., Pavlik, I., Rychlik, I., Mobius, P., Nagata, Y., Damborsky, J.: Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases. Applied and Environmental Microbiology, 2005, 71 (11), 6736-6745.
Kopecna, M., Ondrus, S., Literak, I., Klimes, J., Horvathova, A., Moravkova, M., Bartos, M., Trcka, I., Pavlik, I.: Detection of Mycobacterium avium subsp paratuberculosis in two brown bears in the central European carpathians. Journal of Wildlife Diseases, 2006, 42 (3), 691-695.
Machackova-Kopecna, M., Bartos, M., Straka, M., Ludvik, V., Svastova, P., Alvarez, J., Lamka, J., Trcka, I., Treml, F., Parmova, I., Pavlik, I. : Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis. Veterinary Microbiology, 2005, 105 (3-4), 261-268.
Matlova, L., Dvorska, L., Ayele, W.Y., Bartos, M., Amemori, T., Pavlik, I.: Distribution of Mycobactetium avium complex isolates in tissue samples of pigs fed peat naturally contaminated with mycobacteria as a supplement. Journal of Clinical Microbiology, 2005, 43 (3), 1261-1268.
Moravkova, M., Bartos, M., Dvorska-Bartosova, L., Beran, V., Parmova, I., Ocepek, M., Pate, M., Pavlik, I.: Genetic variability of Mycobacterium avium subsp avium of pig isolates. Veterinarni Medicina, 2007, 52 ( 10), 430-436.
O'Brien, R., Mackintosh, C.G., Bakker, D., Kopecna, M., Pavlik, I., Griffin, J.F.T.: Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp paratuberculosis infection in the red deer (Cervus elaphus). Infection and Immunity, 2006, 74 (6), 3530-3537.
Pate, M., Svara, T., Gombac, M., Paller, T., Zolnir-Dovc, M., Emersic, I., Prodinger, W.M., Bartos, M., Zdovc, I., Krt, B., Pavlik, I., Cvetnic, Z., Pogacnik, M., Ocepek, M.: Outbreak of tuberculosis caused by Mycobacterium caprae in a zoological garden. Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health, 2006, 53 (8), 387-392.
Pavlik, I.: The experience of new European Union Member States concerning the control of bovine tuberculosis. Veterinary Microbiology, 2006, 112 (2-4), 221-230 .
Pavlik, I., Matlova, L., Gilar, M., Bartl, J., Parmova, I., Lysak, F., Alexa, M., Dvorska-Bartosova, L., Svec, V., Vrbas, V., Horvathova, A.: Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003. Veterinarni Medicina, 2007, 52 (9), 392-404.
Pavlik, I., Jahn, P., Moravkova, M., Matlova, L., Treml, F., Cizek, A., Nesnalova, E., Dvorska-Bartosova, L., Halouzka, R.: Lung tuberculosis in a horse caused by Mycobacterium avium subsp avium of serotype 2: a case report. Veterinarni Medicina, 2008, 53 (2), 111-116.
Pisarikova, B., Zraly, Z., Kracmar, S., Trckova, M., Herzig, I.: The use of amaranth (genus Amaranthus L.) in the diets for broiler chickens. Veterinarni Medicina, 2006, 51 (7), 399-407.
Pribylova, R., Pavlik, I., Rozsypalova, Z., Bartos, M.: A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus. European Food Research and Technology, 2006, 223 (1), 139-142.
Pribylova, R., Ptacek, J., Pavlik, I., Bartos, M.: Screening of Solanum tuberosum cultivars for the " ac2" genetic modification. Veterinarni Medicina, 2007, 52 (11), 471-474.
Pribylova, R., Kralik, P., Pisarikova, B., Pavlik, I.: Detection of the antimicrobial peptide gene in different Amaranthus species. Biologia, 2008, 63 (2), 217-220.
Prodinger, W.M., Brandstatter, A., Naumann, L., Pacciarini, M., Kubica, T., Boschiroli, M.L., Aranaz, A., Nagy, G., Cvetnic, Z., Ocepek, M., Skrypnyk, A., Erler, W., Niemann, S., Pavlik, I., Moser, I.: Characterization of Mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping. Journal of Clinical Microbiology, 2005, 43 ( 10), 4984-4992.
Romano, M.I., Amadio, A., Bigi, F., Klepp, L., Etchechoury, I., Llana, M.N., Morsella, C., Paolicchi, F., Pavlik, I., Bartos, M., Leao, S.C., Cataldi, A.: Further analysis of VNTR and MIRU in the genome of Mycobacterium avium complex, and application to molecular epidemiology of isolates from South America. Veterinary Microbiology, 2005, 110 (3-4 ), 221-237.
Shitaye, J.E., Parmova, I., Matlova, L., Dvorska, L., Horvathova, A., Vrbas, V., Pavlik, I.: Mycobacterial and Rhodococcus equi infections in pigs in the Czech Republic between the years 1996 and 2004: the causal factors and distribution of infections in the tissues. Veterinarni Medicina, 2006, 51 (11), 497-511.
Shitaye, J.E., Getahun, B., Alemayehu, T., Skoric, M., Treml, F., Fictum, P., Vrbas, V., Pavlik, I.: A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS6110PCR examination of tissues with tuberculous lesions in cattle in Ethiopia. Veterinarni Medicina, 2006, 51 (11), 512-522.
Shitaye, J.E., Tsegaye, W., Pavlik, I.: Bovine tuberculosis infection in animal and human populations in Ethiopia: a review. Veterinarni Medicina, 2007, 52 (8), 317-332.
Shitaye, J.E., Matlova, L., Horvathova, A., Moravkova, M., Dvorska-Bartosova, L., Treml, F., Lamka, J., Pavlik, I.: Mycobacterium avium subsp avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods. Veterinary Microbiology, 2008, 127 (1-2), 155-164.
Shitaye, J.E., Matlova, L., Horvathova, A., Moravkova, M., Dvorska-Bartosova, L., Trcka, I., Lamka, J., Treml, F., Vrbas, V., Pavlik, I.: Diagnostic testing of different stages of avian tuberculosis in naturally infected hens (Gallus domesticus) by the tuberculin skin and rapid agglutination tests, faecal and egg examinations. Veterinarni Medicina, 2008, 53 (2), 101-110.
Skoric, M., Shitaye, E.J., Halouzka, R., Fictum, P., Trcka, I., Heroldova, M., Tkadlec, E., Pavlik, I.: Tuberculous and tuberculoid lesions in free living small terrestrial mammals and the risk of infection to humans and animals: a review. Veterinarni Medicina, 2007, 52 (4), 144-161.
Trckova, M., Matlova, L., Hudcova, H., Faldyna, M., Zraly, Z., Dvorska, L., Beran, V., Pavlik, I.: Peat as a feed supplement for animals: a review. Veterinarni Medicina, 2005, 50 (8), 361-377.
Trckova, M., Zraly, Z., Matlova, L., Beran, V., Moravkova, M., Svobodova, J., Pavlik, I.: Effects of peat feeding on the performance and health status of fattening pigs and environmentally derived mycobacteria. Veterinarni Medicina, 2006, 51 (12), 533-543.
Trckova, M., Zraly, Z., Bejcek, P., Matlova, L., Beran, V., Horvathova, A., Faldyna, M., Moravkova, M., Shitaye, J.E., Svobodova, J., Pavlik, I.: Effect of feeding treated peat as a supplement to newborn piglets on the growth, health status and occurrence of conditionally pathogenic mycobacteria. Veterinarni Medicina, 2006, 51 (12), 544-554.
Vasickova, P., Dvorska, L., Lorencova, A., Pavlik, I.: Viruses as a cause of foodborne diseases: a review of the literature. Veterinarni Medicina, 2005, 50 (3), 89-104.
Vasickova, P., Psikal, I., Kralik, P., Widen, F., Hubalek, Z., Pavlik, I.: Hepatitis E virus: a review. Veterinarni Medicina, 2007, 52 (9), 365-384.
Zraly, Z., Pisarikova, B., Trckova, M., Herzig, I., Juzl, M., Simeonovova, J.: Effect of lupine and amaranth on growth efficiency, health, and carcass characteristics and meat quality of market pigs. Acta Veterinaria Brno, 2006, 75 (3), 363-372.
Zraly, Z., Pisarikova, B., Trckova, M., Herzig, I., Juzl, M., Simeonovova, J.: The effect of white lupine on the performance, health, carcass characteristics and meat quality of market pigs. Veterinarni Medicina, 2007, 52 (1), 29-41.
Publications over the last three years
Issues Concerning Survival of Viruses on Surfaces
Vasickova P, Pavlik I, Verani M, Carducci AFood and Environmental Virology 2, 2010, 24-34
http://www.springerlink.com/content/h1816858400113l6/fulltext.pdfEpidemiology and characterization of Staphylococcus epidermidis isolates from humans, raw bovine milk and a dairy plant
Jaglic Z, Michu E, Holasova M, Vlkova H, Babak V, Kolar M, Bardon J, Schlegelova JEpidemiology and Infection 138, 2010, 772-782
Mycobacteria in the environment of pig farms in the Czech Republic between 2003 and 2007
Krizova K, Matlova L, Horvathova A, Moravkova M, Beran V, Boisselet T, Babak V, Slana I, Pavlik IVeterinarni Medicina 55, 2010, 55-69
http://www.vri.cz/docs/vetmed/55-2-55.pdfMycobacterium avium subsp avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report
Kriz P, Slana I , Mrlik V, Moravkova M, Kralova A, Krizova K, Pavlik IVeterinarni Medicina 55, 2010, 87-90
http://www.vri.cz/docs/vetmed/55-2-87.pdfSystemic mixed infection in a brown caiman (Caiman crocodilus fuscus) caused by Mycobacterium szulgai and M. chelonae: a case report
Slany M, Knotek Z, Skoric M, Knotkova Z, Svobodova J, Mrlik V, Moravkova M, Pavlik IVeterinarni Medicina 55, 2010, 91-96
http://www.vri.cz/docs/vetmed/55-2-91.pdfIS900 RFLP types of Mycobacterium avium subsp paratuberculosis in faeces and environmental samples on four dairy cattle farms
Pavlik I, Horvathova A, Bartosova L, Babak V, Moravkova MVeterinarni Medicina 55, 2010, 1-9
http://www.vri.cz/docs/vetmed/55-01-1.pdfAnalysis of publications on paratuberculosis from 1995 to 2009 with emphasis on the period from 2005 to 2009
Kaevska M, Hruska KVeterinarni Medicina 55, 2010, 43-54
http://www.vri.cz/docs/vetmed/55-2-43.pdfFirst isolation of a newly described Mycobacterium insubricum from freshwater fish
Slany M, Mrlik V, Kriz P, Pavlik IVeterinary Microbiology 144, 2010, 254-255
Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: a review
Vondruskova H, Slamova R, Trckova M, Zraly Z, Pavlik IVeterinarni Medicina 55, 2010, 199-224
Grass silage contaminated with Mycobacterium avium subspecies paratuberculosis (MAP): a possible source of paratuberculosis infection in ruminants?
Khol JL, Beran V, Kralik P, Trckova M, Pavlik I, Baumgartner WVeterinarni Medicina 55, 2010, 225-232
Detection and genetic characterisation of Hepatitis E virus in Czech pig production herds
Vasickova P, Psikal I, Widen F, Smitalova R, Bendova J, Pavlik I, Kralik PResearch in Veterinary Science 87, 2009, 143-148
Changes in fatty acid profile and iodine content in milk as influenced by the inclusion of extruded rapeseed cake in the diet of dairy cows
Vesely A, Krizova L, Trinacty J, Hadrova S, Navratilova M, Herzig I, Fisera MCzech Journal of Animal Science 54, 2009, 201-209
The effect of kaolin feeding on efficiency, health status and course of diarrhoeal infections caused by enterotoxigenic Escherichia coli strains in weaned piglets
Trckova M, Vondruskova H, Zraly Z, Alexa P, Hamrik J, Kummer V, Maskova J, Mrlik V, Krizova K, Slana I, Leva L, Pavlik IVeterinarni Medicina 54, 2009, 47-63
http://www.vri.cz/docs/vetmed/54-2-47.pdfOccurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants
Stevenson K, Alvarez J, Bakker D, Biet F, de Juan L, Denham S, Dimareli Z, Dohmann K, Gerlach GF, Heron I, Kopecna M, May L, Pavlik I, Sharp JM, Thibault VC, Willemsen P, Zadoks RN, Greig ABmc Microbiology 9, 2009, Article Number 212
http://www.biomedcentral.com/content/pdf/1471-2180-9-212.pdfMycobacterium avium subsp paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCR
Slana I, Liapi M, Moravkova M, Kralova A, Pavlik IPreventive Veterinary Medicine 89, 2009, 223-226
Mycobacterium avium subsp paratuberculosis and M-a. avium Detected by Culture, IS900 and IS901 Highly Sensitive PCR in Bulk Tank Milk from Dairy Herds in the Czech Republic between 2002 and 2004
Slana I, Bartos M, Roubal P, Babak V, Pavlik ICzech Journal of Food Sciences 27, 2009, 372-378
Distribution of Non-Tuberculosis Mycobacteria in Environmental Samples from a Slaughterhouse and in Raw and Processed Meats
Shitaye JE, Horvathova A, Bartosova L, Moravkova M, Kaevska M, Donnelly N, Pavlik ICzech Journal of Food Sciences 27, 2009, 194-202
Mycobacterium avium subsp hominissuis Infection in a Pet Parrot
Shitaye EJ, Grymova V, Grym M, Halouzka R, Horvathova A, Moravkova M, Beran V, Svobodova J, Dvorska-Bartosova L, Pavlik IEmerging Infectious Diseases 15, 2009, 617-619
Comparison of the conventional culture, the manual fluorescent MGIT system and the automated fluorescent MGIT 960 culture system for the detection of Mycobacterium avium ssp avium in tissues of naturally infected hens
Shitaye EJ, Beran V, Svobodova J, Moravkova M, Babak V, Pavlik IFolia Microbiologica 54, 2009, 137-141
Major Mammary Pathogens as Contributors to Total Bacterial Counts in Raw Milk
Rysanek D, Zouharova M, Babak VActa Veterinaria Brno 78, 2009, 455-461
Monitoring major mastitis pathogens at the population level based on examination of bulk tank milk samples
Rysanek D, Zouharova M, Babak VJournal of Dairy Research 76, 2009, 117-123
Oligonucleotide Microarray Technology and its Application to Mycobacterium avium subsp paratuberculosis Research: A Review
Pribylova R, Kralik P, Pavlik IMolecular Biotechnology 42, 2009, 30-40
Detection of Genetic Modification 'ac2' in Potato Foodstuffs
Pribylova R, Kralik P, Pavlik I, Bartos MFood Technology and Biotechnology 47, 2009, 125-130
Nutritional Value of Lupine in the Diets for Pigs (a Review)
Pisarikova B, Zraly ZActa Veterinaria Brno 78, 2009, 399-409
Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP
Pate M, Moravkova M, Krt B, Pavlik I, Ocepek MVeterinarni Medicina 54, 2009, 270-279
http://www.vri.cz/docs/vetmed/54-6-270.pdfApplication of DNA-based Techniques for Intraspecies Differentiation of Hake Fish
Hubalkova Z, Kralik P, Kasalova J, Rencova EActa Veterinaria Brno 78, 2009, 673-U142
The effect of humic acid on zinc accumulation in chicken broiler tissues
Herzig I, Navratilova M, Totusek J, Suchy P, Vecerek V, Blahova J, Zraly ZCzech Journal of Animal Science 54, 2009, 121-127
Distribution of Mycobacterium avium subsp paratuberculosis in tissues of naturally infected cattle as affected by age
Hasonova L, Trcka I, Babak V, Rozsypalova Z, Pribylova R, Pavlik IVeterinarni Medicina 54, 2009, 257-269
http://www.vri.cz/docs/vetmed/54-6-257.pdfEffect of humic acids on lead accumulation in chicken organs and muscles
Zraly Z, Pisarikova B, Trckova M, Navratilova MActa Veterinaria Brno 77, 2008, 439-445
Replacement of soya in pig diets with white lupine cv. Butan
Zraly Z, Pisarikova B, Trckova M, Dolezal M, Thiemel J, Simeonovova J, Juzl MCzech Journal of Animal Science 53, 2008, 418-430
The effect of humic acid on mercury accumulation in chicken organs and muscle tissues
Zraly Z, Pisarikova B, Navratilova MCzech Journal of Animal Science 53, 2008, 472-478
Biofilms and Hygiene on Dairy Farms and in the Dairy Industry: Sanitation Chemical Products and their Effectiveness on Biofilms - a Review
Vlkova H, Babak V, Seydlova R, Pavlik I, Schlegelova JCzech Journal of Food Sciences 26, 2008, 309-323
On-farm spread of Mycobacterium avium subsp paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination
Slana I, Kralik P, Kralova A, Pavlik IInternational Journal of Food Microbiology 128, 2008, 250-257
Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review
Slana I, Paolicchi F, Janstova B, Navratilova P, Pavlik IVeterinarni Medicina 53, 2008, 283-306
http://www.vri.cz/docs/vetmed/53-6-283.pdfFlepatoprotective effects of milk thistle (Silybum marianum) seed cakes during the chicken broiler fattening
Suchy P, Strakova E, Kummer V, Herzig I, Pisakikova V, Blechova R, Maskova JActa Veterinaria Brno 77, 2008, 31-38
Diagnostic testing of different stages of avian tuberculosis in naturally infected hens (Gallus domesticus) by the tuberculin skin and rapid agglutination tests, faecal and egg examinations
Shitaye JE, Matlova L, Horvathova A, Moravkova M, Dvorska-Bartosova L, Trcka I, Lamka J, Treml F, Vrbas V, Pavlik IVeterinarni Medicina 53, 2008, 101-110
http://www.vri.cz/docs/vetmed/53-2-101.pdfMycobacterium avium subsp avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods
Shitaye JE, Matlova L, Horvathova A, Moravkova M, Dvorska-Bartosova L, Treml F, Lamka J, Pavlik IVeterinary Microbiology 127, 2008, 155-164
Resistance to erythromycin of Staphylococcus spp. isolates from the food chain
Schlegelova J, Vlkova H, Babak V, Holasova M, Jaglic Z, Stosova T, Sauer PVeterinarni Medicina 53, 2008, 307-314
http://www.vri.cz/docs/vetmed/53-6-307.pdfThe Biofilm-Positive Staphylococcus epidermidis Isolates in Raw Materials, Foodstuffs and on Contact Surfaces in Processing Plants
Schlegelova J, Babak V, Holasova M, Dendis MFolia Microbiologica 53, 2008, 500-504
Detection of the antimicrobial peptide gene in different Amaranthus species
Pribylova R, Kralik P, Pisarikova B, Pavlik IBiologia 63, 2008, 217-220
Nutritional value of white lupine cultivar Butan in diets for fattening pigs
Pisarikova B, Zraly Z, Bunka F, Trckova MVeterinarni Medicina 53, 2008, 124-134
http://www.vri.cz/docs/vetmed/53-3-124.pdfLung tuberculosis in a horse caused by Mycobacterium avium subsp avium of serotype 2: a case report
Pavlik I, Jahn P, Moravkova M, Matlova L, Treml F, Cizek A, Nesnalova E, Dvorska-Bartosova L, Halouzka RVeterinarni Medicina 53, 2008, 111-116
http://www.vri.cz/docs/vetmed/53-2-111.pdfTiming of breeding, habitat preference and reproductive success of marsh harriers (Circus aeruginosus)
Nemeckova I, Mrlik V, Drozd PBiologia 63, 2008, 261-265
A mixed infection of Mycobacterium avium subsp paratuberculosis and M-a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report
Moravkova M, Trcka I, Lamka J, Pavlik IVeterinarni Medicina 53, 2008, 445-451
http://www.vri.cz/docs/vetmed/53-8-445.pdfStrategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues
Moravkova M, Hlozek P, Beran V, Pavlik I, Preziuso S, Cuteri V, Bartos MResearch in Veterinary Science 85, 2008, 257-264
Estrogenic activity of environmental polycyclic aromatic hydrocarbons in uterus of immature Wistar rats
Kummer V, Maskova J, Zraly Z, Neca J, Simeckova P, Vondracek J, Machala MToxicology Letters 180, 2008, 212-221
Distribution and transmission of Mycobacterium avium subspecies paratuberculosis in farmed red deer (Cervus elaphus) studied by faecal culture, serology and IS900 RFLP examinations
Kopecna M, Parmova I, Dvorska-Bartosova L, Moravkova M, Babak V, Pavlik IVeterinarni Medicina 53, 2008, 510-523
http://www.vri.cz/docs/vetmed/53-9-510.pdfEvaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum
Kohler H, Burkert B, Pavlik I, Diller R, Geue L, Conraths FJ, Martin GBerliner und Munchener Tierarztliche Wochenschrift 121, 2008, 203-210
The wildlife hosts of Mycobacterium avium subsp paratuberculosis in the Czech Republic during the years 2002-2007
Kopecna M, Trcka I, Lamka J, Moravkova M, Koubek P, Heroldova M, Mrlik V, Kralova A, Pavlik IVeterinarni Medicina 53, 2008, 420-426
http://www.vri.cz/docs/vetmed/53-8-420.pdfExperimental study of pathogenicity of Pasteurella multocida serogroup F in rabbits
Jaglic Z, Jeklova E, Leva L, Kummer V, Kucerova Z, Faldyna M, Maskova J, Nedbalcova K, Alexa PVeterinary Microbiology 126, 2008, 168-177
Dexamethasone-induced immunosuppression: a rabbit model
Jeklova E, Leva L, Jaglic Z, Faldyna MVeterinary Immunology and Immunopathology 122, 2008, 231-240
Identification of gadoid species in fish meat by polymerase chain reaction (PCR) on genomic DNA
Hubalkova Z, Kralik P, Kasalova J, Rencova EJournal of Agricultural and Food Chemistry 56, 2008, 3454-3459
Lawsonia intracellularis in rodents from pig farms with the occurrence of porcine proliferative enteropathy
Friedman M, Bednar V, Klimes J, Smola J, Mrlik V, Literak ILetters in Applied Microbiology 47, 2008, 117-121
The effect of white lupine on the performance, health, carcass characteristics and meat quality of market pigs
Zraly Z, Pisarikova B, Trckova M, Herzig I, Juzl M, Simeonovova JVeterinarni Medicina 52, 2007, 29-41
http://www.vri.cz/docs/vetmed/52-1-29.pdfHepatitis E virus: a review
Vasickova P, Psikal I, Kralik P, Widen F, Hubalek Z, Pavlik IVeterinarni Medicina 52, 2007, 365-384
http://www.vri.cz/docs/vetmed/52-9-365.pdfSelenium content in the blood serum and urine of ewes receiving selenium-enriched unicellular alga Chlorella
Travnicek J, Pisek L, Herzig I, Doucha J, Kvicala J, Kroupova V, Rodinova HVeterinarni Medicina 52, 2007, 42-48
http://www.vri.cz/docs/vetmed/52-1-42.pdfTuberculous and tuberculoid lesions in free living small terrestrial mammals and the risk of infection to humans and animals: a review
Skoric M, Shitaye EJ, Halouzka R, Fictum P, Trcka I, Heroldova M, Tkadlec E, Pavlik IVeterinarni Medicina 52, 2007, 144-161
http://www.vri.cz/docs/vetmed/52-4-144.pdfBulk tank milk somatic cell count and sources of raw milk contamination with mastitis pathogens
Rysanek D, Babak V, Zouharova MVeterinarni Medicina 52, 2007, 223-230
http://www.vri.cz/docs/vetmed/52-6-223.pdfBovine tuberculosis infection in animal and human populations in Ethiopia: a review
Shitaye JE, Tsegaye W, Pavlik IVeterinarni Medicina 52, 2007, 317-332
http://www.vri.cz/docs/vetmed/52-8-317.pdfScreening of Solanum tuberosum cultivars for the " ac2" genetic modification
Pribylova R, Ptacek J, Pavlik I, Bartos MVeterinarni Medicina 52, 2007, 471-474
http://www.vri.cz/docs/vetmed/52-11-471.pdfThe content of insoluble fibre and crude protein value of the aboveground biomass of Amaranthus cruentus and A-hypochondriacus
Pisarikova B, Peterka J, Trckova M, Moudry J, Zraly Z, Herzig ICzech Journal of Animal Science 52, 2007, 348-353
Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003
Pavlik I, Matlova L, Gilar M, Bartl J, Parmova I, Lysak F, Alexa M, Dvorska-Bartosova L, Svec V, Vrbas V, Horvathova AVeterinarni Medicina 52, 2007, 392-404
http://www.vri.cz/docs/vetmed/52-9-392.pdfGenetic variability of Mycobacterium avium subsp avium of pig isolates
Moravkova M, Bartos M, Dvorska-Bartosova L, Beran V, Parmova I, Ocepek M, Pate M, Pavlik IVeterinarni Medicina 52, 2007, 430-436
http://www.vri.cz/docs/vetmed/52-10-430.pdfEffect of postnatal exposure to benzo[a]pyrene on the uterus of immature rats
Kummer V, Maskova J, Zraly Z, Matiasovic J, Faldyna MExperimental and Toxicologic Pathology 59, 2007, 69-76
Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review
Hubalkova Z, Kralik P, Tremlova B, Rencova EVeterinarni Medicina 52, 2007, 273-292
http://www.vri.cz/docs/vetmed/52-7-273.pdfComparative evaluation of positive tests to Mycobacterium avium subsp paratuberculosis in clinically healthy sheep and goats in South-West Greece using molecular techniques, serology, and culture
Ikonomopoulos J, Balaskas C, Kantzoura B, Fragiadaki E, Pavlik I, Bartos M, Lukas JC, Gazouli MVeterinary Journal 174, 2007, 337-343
Model trial investigating retention in selected tissues using broiler chicken fed cadmium and humic acid
Herzig I, Navratilova M, Suchy P, Vecerek V, Totusek JVeterinarni Medicina 52, 2007, 162-168
http://www.vri.cz/docs/vetmed/52-4-162.pdfContent of iodine in broiler meat
Herzig I, Travnicek J, Kursa V, Kroupova J, Reznicek IActa Veterinaria Brno 76, 2007, 137-141
Comparison of methods for the determination of antimicrobial resistance in Campylobacter spp. human and the food chain isolates
Holasova M, Karpiskova R, Karpiskova S, Babak V, Schlegelova JVeterinarni Medicina 52, 2007, 169-174
http://www.vri.cz/docs/vetmed/52-4-169.pdfAvian tuberculosis in naturally infected captive water birds of the Ardeideae and Threskiornithidae families studied by serotyping, IS901 RFLP typing, and virulence for poultry
Dvorska L, Matlova L, Ayele WY, Fischer OA, Amemori T, Weston RT, Alvarez J, Beran V, Moravkova M, Pavlik IVeterinary Microbiology 119, 2007, 366-374
http://www.vri.cz/news/prilohy/pril517.pdfDetermination of volatile organic compounds in the crude and heat treated amaranth samples
Ciganek M, Pisarikova B, Zraly ZVeterinarni Medicina 52, 2007, 111-120
http://www.vri.cz/docs/vetmed/52-3-111.pdfPrevalence of Neospora caninum and Toxoplasma gondii antibodies in wild ruminants from the countryside or captivity in the Czech Republic
Bartova E, Sedlak K, Pavlik I, Literak IJournal of Parasitology 93, 2007, 1216-1218